Buy Guided BIOS 390 Week 6 LAB Isolate Plasmid DNA
Once a plasmid has … introduced into competent bacterial cells and the cells have grown into colonies on a medium selective for cells containing the plasmid, the next step is to perform a miniprep of the plasmid DNA in preparation for DNA sequencing. The three steps are 1) growing cells in liquid culture; 2) purifying the plasmid DNA from the culture, and 3) performing a restriction digest on the purified DNA to determine whether the DNA insert in the plasmid is the … size. To start the liquid culture, cells from an isolated bacterial colony are … in a selective medium (nutrient broth with an antibiotic to which the plasmid provides resistance). ……….. document continue
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