Buy Solved CHEM 120 Unit 7 Labs – OL Lab 12 and OL Lab 13

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Buy Solved CHEM 120 Unit 7 Labs-OL Lab 12 and OL Lab 13

 

CHEM 120 Unit 7 Lab: OL Lab 12: Introduction to Food Macromolecules

Learning Objectives

  • Identify the types of macromolecules found in food
  • Identify the structure of carbohydrates, proteins, and lipids
  • Detect macromolecules in food samples

Introduction
Macromolecules are very large molecules created by the polymerization of small units called monomers. Most of the macromolecules are present in everyday life, for instance in food.

Learn about biological macromolecules
There are several types of biological macromolecules: carbohydrates, proteins, lipids, and nucleic acids. All macromolecules, except lipids, are polymers. A polymer is a long molecule composed of chains of monomers. Monomers are small molecules that serve as building blocks of polymers. In addition, there are also oligomers in nature. Oligomers are molecular complexes composed of a few monomer units, instead of the theoretically unlimited number of monomers. Dimers and trimers are oligomers composed of two and three monomers, respectively, such as the lactose in milk for instance. However, in biochemistry, an oligomer usually refers to a macromolecular complex formed by the non-covalent bonding of a few macromolecules, such as nucleic acids or proteins. An example is the oligomers found in many neurodegenerative diseases, such as the alpha-synuclein aggregations in Parkinson’s disease.

Help your friend with your macromolecule knowledge
In the Introduction to Food Macromolecules simulation, you will help your friend get a healthy diet and investigate the types of macromolecules found in food. By performing a series of biochemistry tests, you will know the contents of various food items.

Can you use your macromolecule knowledge to convince your friend to change her diet to a healthier one?

Part 1: Complete Labster Lab: Introduction to Food Macromolecules 

Part 2: Report and Reflection 

Purpose: Describe in complete sentences and in your own words, the purpose of this experiment.

Observations: Record three observations from the simulation.

Answer the questions below:

  1. Describe the key functions of one of the cases of biomolecules you studied in this virtual lab AND include key structural details.
  2. Choose food in your house. What are some of the biomolecules you expect to be in this food and why?
  3. Reflection: Consider what you learned from this simulation. Reflect on three to four key concepts that you learned in this lab exercise. How could the lessons learned in this virtual lab be related to a real-world situation in the community/world or your future career?  Be specific in your answer (this should require 5-10 sentences).

CHEM 120 Unit 7 Lab: OL Lab 13: Protein Synthesis

Learning Objectives

  • Explain the translation process from mRNA to amino acid
  • … post-translational modification of proteins
  • Explain protein synthesis processing in the ribosome
  • … primary, secondary, tertiary, and quaternary structures of protein
  • Explain the basic principles of mass spectrometry (MALDI-TOF)

Introduction
In the Protein Synthesis lab, you will learn about the difference between protein synthesis in prokaryotes (using E. coli) and eukaryotes (using Chinese hamster ovarian (CHO) cells).

Prepare recombinant Erythropoietin and use the mass spectrometer
Your first task in the lab will … to prepare recombinant erythropoietin (EPO) that is … into E. coli and CHO cells. The lab assistant will prepare the recombinant EPO and you will measure the mass-to-charge ratio … a mass spectrometer. Not sure how to handle the mass spectrometer? No worries! You can just take out your lab pad and find an animated video to learn the basics.

Study the translation process from mRNA to amino acids
You will also learn about the translation process from mRNA to amino acids and how amino acids are … to proteins. A 3D animation will describe how codons are translated into amino acids, how these amino acids are … together by peptide bonds creating a primary structure of the protein, and how the primary structure is … into the secondary, tertiary, and quaternary structure.

Investigate doping in bike athletes
In the last part of the Protein Synthesis lab, you will use mass spectrometry to investigate if any athletes are using recombinant human erythropoietin (rhEPO) as a doping substance. You will do so by collaborating with the doping agent who collects urine samples at a large bicycle race.

Will you be able to detect if any of the athletes are using doping?

Part 1: Complete Labster Lab: Protein Synthesis

Part 2: Report and Reflection
Purpose
: Describe in complete sentences and in your own words, the purpose of this experiment.

Observations: Record three observations from the simulation.

Answer the questions below:

  1. In your own words, describe the process of gene expression beginning from the nucleus to the formation of the polypeptide sequence.
  2. Complete the table below:
  3. Assume that RNA Polymerase will read the parental strand of DNA given here and write the mRNA sequence that would result: – TATGCTTCCGTA –
  4. Reflection: Consider what you learned from this simulation. Reflect on three to four key concepts that you learned in this lab exercise. How could the lessons learned in this virtual lab relate to a real-world situation in the community/world or your future career?  Be specific in your answer (this should require 5-10 sentences).

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